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Mrc1 Antibody (OASA05048)
| Datasheets/Manuals | Printable datasheet for Mrc1 Antibody (OASA05048) |
|---|
| Predicted Species Reactivity | Mouse |
|---|---|
| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | MR5D3 |
| Isotype | IgG2a |
| Host | Rat |
| Application | Flow cytometry|Immunofluorescence|Immunohistochemistry-Frozen|Immunoprecipitation |
| Additional Information | Fusion Partners: Spleen cells from immunized Fischer rats were fused with cells of the Y3 myeloma cell line. Carrier Free: Yes |
| Reconstitution and Storage | -20°C |
| Immunogen | Chimaeric CRD4-7-Fc protein |
| Purification | Protein G Purified |
| Concentration | 1 mg/ml |
| Specificity | Rat anti mouse CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a 175 kDa type 1 membrane glycoprotein that is also known as CD206. |
| Application Info | FC: 1/10-1/20 (Use 10 uL of the suggested working dilution to label 106 cells in 100 uL.) CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Optimal dilutions should be determined by the end user. |
| Publications | Cathepsin B-Mediated Activation of Trypsinogen in Endocytosing Macrophages Increases Severity of Pancreatitis in Mice. Gastroenterology. 154, 704-718.e10 (2018). 29079517 Lysosome associated membrane proteins maintain pancreatic acinar cell homeostasis: LAMP-2 deficient mice develop pancreatitis. Cell Mol Gastroenterol Hepatol. 1, 678-694 (2015). 26693174 NLRP3 Inflammasome Regulates Development of Systemic Inflammatory Response and Compensatory Anti-Inflammatory Response Syndromes in Mice With Acute Pancreatitis. Gastroenterology. 158, 253-269.e14 (2020). 31593700 |
| Description |
| Gene Symbol | Mrc1 |
|---|---|
| Alias Symbols | M, MR, CD206, AW259686 |
| NCBI Gene Id | 17533 |
| Protein Name | Macrophage mannose receptor 1 |
| Description of Target | CD206 is expressed on most tissue macrophages, certain endothelial cells and in vitro derived dendritic cells
The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognizes carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins. Rat anti mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands. Clone MR5D3 has also been shown to work in Western Blot. |
| Uniprot ID | Q61830 |
| Protein Accession # | NP_032651.2 |
| Nucleotide Accession # | NM_005625 |
| Molecular Weight | 175 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Mrc1 Antibody (OASA05048)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse".
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How long will it take to receive "Mrc1 Antibody (OASA05048)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Mrc1 Antibody (OASA05048)" provided in?
This item is provided in "Liquid. Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Mrc1 Antibody (OASA05048)"?
This target may also be called "M, MR, CD206, AW259686" in publications.
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What is the shipping cost for "Mrc1 Antibody (OASA05048)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Mrc1 Antibody (OASA05048)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Mrc1 Antibody (OASA05048)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "175 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Mrc1 Antibody (OASA05048)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MRC1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MRC1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MRC1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MRC1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MRC1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MRC1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
