Catalog No: OKCD00816 (Formerly GWB-KBBYX2)
Size:96WELLS
Price: $650.00
SKU
OKCD00816
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Datasheets/ManualsPrintable datasheet for MPZ ELISA Kit (Human) (OKCD00816)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Zero, Myelin (MPZ). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Zero, Myelin (MPZ). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Zero, Myelin (MPZ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Zero, Myelin (MPZ) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Zero, Myelin (MPZ) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Zero, Myelin (MPZ) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MPZ Microplate96 Wells (12 x 8 Well Strips)
MPZ Lyophilized Standard2 x 20 ng
100X Biotinylated MPZ Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.117 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Zero, Myelin (MPZ). No significant cross-reactivity or interference between Protein Zero, Myelin (MPZ) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMPZ
Gene Full Namemyelin protein zero
Alias SymbolsCharcot-Marie-Tooth neuropathy 1B;CHM;CHN2;CMT1;CMT1B;CMT2I;CMT2J;CMT4E;CMTDI3;CMTDID;DSS;HMSNIB;MPP;myelin peripheral protein;myelin protein P0;Myelin protein zero;P0.
NCBI Gene Id4359
Protein NameMyelin protein P0
Description of TargetIs an adhesion molecule necessary for normal myelination in the peripheral nervous system. It mediates adhesion between adjacent myelin wraps and ultimately drives myelin compaction.
Uniprot IDP25189
Protein Accession #NP_000521
Nucleotide Accession #NM_000530.7
Protein Size (# AA)248
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