Catalog No: OKDD00401 (Formerly GWB-KBBGB8)
Size:96 Wells
Price: $800.00
SKU
OKDD00401
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Datasheets/ManualsPrintable datasheet for MPI ELISA Kit (Human) (OKDD00401)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology MPI ELISA Kit (Human) (OKDD00401) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for MPI has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MPI is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MPI captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MPI were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MPI were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-MPI Microplate96 Wells (12 x 8 Well Strips)
MPI Lyophlized Standard2 x 10 ng
Assay Diluent1 x 45 mL
100X MPI Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates
Sensitivity< 0.061 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of MPI. No significant cross-reactivity or interference between MPI and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolMPI
Gene Full Namemannose phosphate isomerase
Alias SymbolsCDG1B;mannose-6-phosphate isomerase;phosphohexomutase;Phosphomannose isomerase;phosphomannose isomerase 1;PMI;PMI1.
NCBI Gene Id4351
Protein NameMannose-6-phosphate isomerase
Description of TargetInvolved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.
Uniprot IDP34949
Protein Accession #NP_001276085.1
Nucleotide Accession #NM_001289156.1
Protein Size (# AA)423
Molecular Weight47 kDa
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