- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OAAB10560 |
---|
Predicted Species Reactivity | Human, Mouse |
---|---|
Product Format | Liquid. PBS with 0.09% (W/V) sodium azide. |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB |
Additional Information | Function: Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion. Cellular Location: Endomembrane system; Peripheral membrane protein (By similarity). Cytoplasm (By similarity). Tissue Location: In contrast with the ubiquitous expression of the other JAKs, JAK3 is predominantly expressed in hematopoietic tissues. |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This Mouse Jak3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-161 amino acids from the N-terminal region of mouse Jak3. |
Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
Intended Use | For research use only and not for use in diagnostic or therapeutic procedures. |
Application Info | WB: 1:1000 |
Gene Symbol | Jak3 |
---|---|
Alias Symbols | fae, wil |
NCBI Gene Id | 16453 |
Protein Name | Tyrosine-protein kinase JAK3 |
Description of Target | Tyrosine kinase of the non-receptor type, involved in the interleukin-2 and interleukin-4 signaling pathway. Phosphorylates STAT6, IRS1, IRS2 and PI3K. |
Uniprot ID | Q62137 |
Protein Accession # | NP_001177759.1 |
Molecular Weight | 122641 Da |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "Mouse Jak3 Antibody - N-terminal region (OAAB10560)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".
-
How long will it take to receive "Mouse Jak3 Antibody - N-terminal region (OAAB10560)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
-
What buffer format is "Mouse Jak3 Antibody - N-terminal region (OAAB10560)" provided in?
This item is provided in "Liquid. PBS with 0.09% (W/V) sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Mouse Jak3 Antibody - N-terminal region (OAAB10560)"?
This target may also be called "fae, wil" in publications.
-
What is the shipping cost for "Mouse Jak3 Antibody - N-terminal region (OAAB10560)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "Mouse Jak3 Antibody - N-terminal region (OAAB10560)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "Mouse Jak3 Antibody - N-terminal region (OAAB10560)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "122641 Da".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Mouse Jak3 Antibody - N-terminal region (OAAB10560)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "JAK3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "JAK3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "JAK3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "JAK3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "JAK3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "JAK3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.