MOUSE ANTI DOG IgE Antibody (0.25mg)

Catalog#: OASA00637

• Size: 0.25mg

• Description of Target: MOUSE ANTI DOG IgE
• Alias Symbols: N/A
• Host: Mouse
• Specificity: IgE
• Application: ELISA, WB
• Clone: E6-2A1
• Immunogen: Affinity purified IgE preparation from heavily parasitized and allergic dog serum.
• Product Format: Purified IgG - liquid
• Isotype: IgG2a
• Applications Info: ELISA : The antibody may be used as a capture antibody in a sandwich ELISA in combination with OASA041786B as the detection antibody.
• Predicted Homology Based on Immunogen Sequence: Dog
  Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
  
  Preservative Stabilisers: 0.05% Sodium Azide (NaN₃)
  Approx Protein Conc: IgG concentration 1.0 mg/ml
  
  Buffer Solutions: Phosphate buffered saline pH7.2
• Reconstitution and Storage: Store at +4^oC or at -20^oC if preferred.
  
  This product should be stored undiluted.
  
  Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
• Key Reference: 1. Lin, T. Y., Rush, L. J. and London, C. A. (2006) Generation and characterization of bone marrow-derived cultured canine mast cells. Vet Immunol Immunopathol. 13: 37-52.
  2. Lin, T. Y. and London, C. A. (2006) A functional comparison of canine and murine bone marrow derived cultured mast cells.

Product Protocol: MOUSE ANTI DOG IgE antibody used to evaluate airway responses of dogs with ragweed and ascaris hypersensitivity (OASA00637)

Specificity: IgE
Product Page: MOUSE ANTI DOG IgE antibody (OASA00637)
Antibody Pair Available: OASA00637 Capture OASA00639 Detection
Experiment Type: Respiratory Secretions (RS)

Protocol:
The bronchial washings were concentrated in dialysis tubing to a volume of 1 ml. The RS from ditferent washings were standardized to approximately the same concentration by both the optical density as determined by a Beckman DU spectrophotometer at 280 nm and by their IgA content. For this procedure, rabbit anticanine IgA was prepared and Mancini radial immunodifFusion plates were poured. Prior to use, the RS samples were concentrated or diluted to the same IgA ring diameter as a standard RS used for comparison using techniques.

Summary:
1. Patterson et al. observed that respiratory responses to ascaris antigen could be induced in dogs in whom airway responses did not occur at the time of initial antigen challenge. 2. The results demonstrate that reaginic antibodies against SAA can be detected in the RS of dogs sensitive to ascaris. In contrast, no reaginic antibodies were detected in the RS of dogs sensitive to ragweed, although the serum reaginic antibody titers of these were of similar magnitude to those sensitive to ascaris.

References:
1: Patterson R, Mellies CJ, Kelly JF, Harris KE. Airway responses of dogs with ragweed and ascaris hypersensitivity. Chest. 1974 May;65(5):488-92. PubMed PMID: 4826021.