Catalog No: OKEH02344
Size:96T
Price: $625.00
SKU
OKEH02344
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MNAT1 ELISA Kit (Human) (OKEH02344) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MNAT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MNAT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MNAT1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 6.8% (n = 20)
ELISA Kit Component
ComponentAmount
MNAT1 Microplate96 Wells (12 x 8 Well strips)
MNAT1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated MNAT1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity34 pg/mL
SpecificityNatural and recombinant Human CDK-activating kinase assembly factor MAT1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMNAT1
Gene Full NameMNAT1 component of CDK activating kinase
Alias SymbolsCAP35;CDK7/cyclin-H assembly factor;CDK-activating kinase assembly factor MAT1;cyclin G1 interacting protein;Cyclin-G1-interacting protein;MAT1;Menage a trois;menage a trois 1 (CAK assembly factor);menage a trois homolog 1, cyclin H assembly factor;menage a trois-like protein 1 cyclin H assembly factor;MNAT CDK-activating kinase assembly factor 1;MNAT1, CDK activating kinase assembly factor;p35;p36;RING finger protein 66;RING finger protein MAT1;RNF66;TFB3.
NCBI Gene Id4331
Protein NameP51948
Description of TargetCDK-activating kinase assembly factor MAT1
Uniprot IDhttps://www.uniprot.org/uniprot/P51948
Protein Accession #NP_001171434.1
Nucleotide Accession #NM_001177963.1
Protein Size (# AA)309
Molecular Weight36 kDa
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