MMRN2 ELISA Kit (Mouse) (OKCD00979)

Catalog No: OKCD00979 (Formerly GWB-KBBER7)

Size:96WELLS

Mouse Mmrn2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Multimerin 2 (MMRN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Multimerin 2 (MMRN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Multimerin 2 (MMRN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Multimerin 2 (MMRN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Multimerin 2 (MMRN2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Multimerin 2 (MMRN2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Mmrn2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Mmrn2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Mmrn2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.134 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Multimerin 2 (MMRN2). No significant cross-reactivity or interference between Multimerin 2 (MMRN2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Mmrn2
• Gene Full Name: multimerin 2
• Alias Symbols: AA986839;elastin microfibril interfacer 3;Emi;Emilin3;ENDOGL;EndoGly;EndoGlyx-1;ENDOGLYX1;multimerin-2.
• NCBI Gene Id: 105450
• Protein Name: Multimerin-2
• Description of Target: Inhibits endothelial cells motility and acts as a negative regulator of angiogenesis; it downregulates KDR activation by binding VEGFA.
• Uniprot ID: A6H6E2
• Protein Accession #: NP_694767
• Nucleotide Accession #: NM_153127.3
• Protein Size (# AA): 943