MMP9 Antibody - C - terminal region (OABB00316)

Catalog No: OABB00316 (Formerly GWB-BBP238)

Size:100UG

Lane 1: Rat Embryo Tissue Lysate
Lane 2: MM453 Cell Lysate
Lane 3: Hela Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 5: Jurkat Cell Lysate
Lane 6: HT1080 Cell Lysate

IHC(P): Human Lung Cancer Tissue

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Hamster|Human
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Western blot
• Additional Information: Notes: WB: The detection limit for MMP-9 is approximately 0.5ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Matrix metallopeptidase 9(MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B(GELB), is an enzyme that in humans is encoded by the MMP9 gene. Proteins of the matrix metalloproteinase(MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat
• Reference: 1.Template:, 92kDa type IV collagenase)
  2.Yuichiro Hirose et al. (May 2008). "A Functional Polymorphism in THBS2 that Affects Alternative Splicing and MMP Binding Is Associated with Lumbar-Disc Herniation".
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Matrix metalloproteinase-9(MMP9) detection. Tested with WB in Human;Rat.

Target Info

• Gene Symbol: MMP9
• Gene Full Name: matrix metallopeptidase 9
• Alias Symbols: 92 kDa gelatinase;92 kDa type IV collagenase;CLG4B;Gelatinase B;GELB;macrophage gelatinase;MANDP2;matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase);matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase);matrix metalloproteinase-9;MMP-9;type V collagenase.
• NCBI Gene Id: 4318
• Protein Name: Matrix metalloproteinase-9
• Description of Target: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
• Uniprot ID: P14780
• Molecular Weight: 78458 MW