Catalog No: OKEH00176
Size:96T
Price: $425.00
SKU
OKEH00176
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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MMP2 ELISA Kit (Human) : 96 Wells (OKEH00176)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MMP2 ELISA Kit (Human) (OKEH00176) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP2 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 8.7% (n = 20)
ELISA Kit Component
ComponentAmount
MMP2 Microplate96 Wells (12 x 8 Well strips)
MMP2 Lyophilized Standard2
100X Biotinylated MMP2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human 72 kDa type IV collagenase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMMP2
Gene Full Namematrix metallopeptidase 2
Alias Symbols72 kDa gelatinase;72 kDa type IV collagenase;CLG4;CLG4A;collagenase type IV-A;Gelatinase A;matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase);matrix metalloproteinase-2;matrix metalloproteinase-II;MMP-2;MMP-II;MONA;neutrophil gelatinase;TBE-1.
NCBI Gene Id4313
Protein NameP08253
Description of Target72 kDa type IV collagenase
Uniprot IDhttps://www.uniprot.org/uniprot/P08253
Protein Accession #NP_001121363
Nucleotide Accession #NM_001127891
Protein Size (# AA)660
Molecular Weight74 kDa
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