MMP15 ELISA Kit (Mouse) (OKCD01189)

Catalog No: OKCD01189 (Formerly GWB-KBBGX3)

Size:96WELLS

Mouse Mmp15 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	81-91%	89-103%	81-88%	84-98%
  heparin plasma(n=5)	83-104%	92-101%	80-91%	78-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 15 (MMP15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 15 (MMP15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 15 (MMP15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Matrix Metalloproteinase 15 (MMP15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	83-103	87
  heparin plasma(n=5)	91-104	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 15 (MMP15) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 15 (MMP15) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Mmp15 Microplate: 96 Wells (12 x 8 Well Strips)
  • Mmp15 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated Mmp15 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 31 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 15 (MMP15). No significant cross-reactivity or interference between Matrix Metalloproteinase 15 (MMP15) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Mmp15
• Gene Full Name: matrix metallopeptidase 15
• Alias Symbols: AI503551;matrix metalloproteinase-15;Membrane type 2-MMP;Membrane-type matrix metalloproteinase 2;membrane-type-2 matrix metalloproteinase;MT2-MM;MT2-MMP;MT-MMP 2.
• NCBI Gene Id: 17388
• Protein Name: Matrix metalloproteinase-15
• Description of Target: Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.
• Uniprot ID: O54732
• Protein Accession #: NP_032635
• Nucleotide Accession #: NM_008609.4
• Protein Size (# AA): 657