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Catalog No: OKCD01313
Size:96WELLS
Price: $725.00
SKU
OKCD01313
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MMP1 High Sensitivity ELISA Kit (Rabbit) (OKCD01313)

Datasheets/ManualsPrintable datasheet for MMP1 High Sensitivity ELISA Kit (Rabbit) (OKCD01313)
Product Info
Predicted Species ReactivityOryctolagus cuniculus|Rabbit
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)99-105%87-94%82-90%86-97%
Heparin Plasma (n=5)94-101%81-93%92-102%98-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Matrix Metalloproteinase 1 (MMP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Matrix Metalloproteinase 1 (MMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Matrix Metalloproteinase 1 (MMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Matrix Metalloproteinase 1 (MMP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)95-104101
Heparin Plasma (n=5)78-10394
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Matrix Metalloproteinase 1 (MMP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Matrix Metalloproteinase 1 (MMP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MMP1 Microplate96 Wells (12 x 8 Well Strips)
MMP1 Lyophilized Standard2
100X Biotinylated MMP1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity11.5 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 1 (MMP1). No significant cross-reactivity or interference between Matrix Metalloproteinase 1 (MMP1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMMP1
Gene Full Namematrix metallopeptidase 1
Alias Symbolscollagenase-1;interstitial collagenase;matrix metallopeptidase 1 (interstitial collagenase);matrix metalloproteinase-1;MMP-1.
NCBI Gene Id100009110
Protein NameInterstitial collagenase
Description of TargetCleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X.
Uniprot IDP13943
Protein Accession #NP_001164610.1
Nucleotide Accession #NM_001171139.2
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