MMP1 ELISA Kit (Pig) (OKEH03807)

Catalog No: OKEH03807

Size:96T

Pig MMP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology MMP1 ELISA Kit (Pig) (OKEH03807) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Component:
  • Anti-MMP1 Microplate: 96 Wells (12 x 8 Well strips)
  • MMP1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated MMP1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Pig Interstitial collagenase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MMP1
• Gene Full Name: matrix metallopeptidase 1
• Alias Symbols: interstitial collagenase;matix metalloproteinase I collagenase propeptide;matrix metallopeptidase 1 (interstitial collagenase);matrix metalloproteinase 1 (type I collagenase);matrix metalloproteinase-1;MMP-1;Mmp1a.
• NCBI Gene Id: 397320
• Protein Name: P21692
• Description of Target: Interstitial collagenase
• Uniprot ID: https://www.uniprot.org/uniprot/P21692
• Protein Accession #: NP_001159701.1
• Nucleotide Accession #: NM_001166229.1
• Protein Size (# AA): 469
• Molecular Weight: 54 kDa