Catalog No: OKBB00473
Size:96 Wells
Price: $526.00
SKU
OKBB00473
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human MMP-12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-12 has been precoated onto 96-well plates. Standards(NS0, L17-C470) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human MMP-12 amount of sample captured in plate.
ELISA Kit Range62.5 pg/ml - 4,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)4781512238852617242653
SD28.6884.7107.537.9113.8143.3
CV%65.64.57.26.65.4
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human MMP-12 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human MMP-12 Standard2 x 10 ng/tube
100X Biotinylated Anti-Human MMP-12 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human MMP-12
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: L17-C470
DescriptionFor quantitative detection of human MMP-12 in cell culture supernatants, serum and plasma(heparin).
Gene SymbolMMP12
Gene Full Namematrix metallopeptidase 12 (macrophage elastase)
Alias SymbolsEC 3.4.24.65, HME, Macrophage elastase, Macrophage metalloelastase, Macrophage metaloelastase, Matrix metallopeptidase 12 (macrophage elastase), Matrix metalloprotease 12, Matrix metalloproteinase-12, MGC138506, MME, MMP 12, MMP-12, Mmp12, MMP12_HUMAN
NCBI Gene Id4321
Protein NameMacrophage metalloelastase
Description of TargetMatrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.
Uniprot IDP39900
Protein Accession #NP_002417.2
Nucleotide Accession #NM_002426.4
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