MMD ELISA Kit (Human) (OKCD00282)

Catalog No: OKCD00282 (Formerly GWB-KBBMF3)

Size:96WELLS

Human MMD ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monocyte To Macrophage Differentiation Associated Protein (MMA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monocyte To Macrophage Differentiation Associated Protein (MMA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monocyte To Macrophage Differentiation Associated Protein (MMA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Monocyte To Macrophage Differentiation Associated Protein (MMA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte To Macrophage Differentiation Associated Protein (MMA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte To Macrophage Differentiation Associated Protein (MMA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MMD Microplate: 96 Wells (12 x 8 Well Strips)
  • MMD Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated MMD Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Monocyte To Macrophage Differentiation Associated Protein (MMA). No significant cross-reactivity or interference between Monocyte To Macrophage Differentiation Associated Protein (MMA) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MMD
• Gene Full Name: monocyte to macrophage differentiation associated
• Alias Symbols: macrophage maturation-associated;MMA;MMD1;monocyte to macrophage differentiation factor;monocyte to macrophage differentiation protein;PAQR11;progestin and adipoQ receptor family member 11;progestin and adipoQ receptor family member XI.
• NCBI Gene Id: 23531
• Protein Name: Monocyte to macrophage differentiation factor
• Description of Target: Involved in the dynamics of lysosomal membranes associated with microglial activation following brain lesion.
• Uniprot ID: Q15546
• Protein Accession #: NP_036461
• Nucleotide Accession #: NM_012329.2
• Protein Size (# AA): 238