MLL2 Antibody (OAEB00224)

Catalog No: OAEB00224 (Formerly GWB-BBD6E2)

Size:100UG

OAEB00224 (4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

OAEB00224 (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: KMT2D
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human: 84%
• Peptide Sequence: DSQNLAGEDKDSQ-C
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:16000.
  Western Blot: Preliminary experiments gave bands at approx 75kDa and 25kDa in lysates of cell line K562 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 593kDa according to NP_003473.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
• Reference: * Prasad R, Zhadanov AB, Sedkov Y, Bullrich F, Druck T, Rallapalli R, Yano T, Alder H, Croce CM, Huebner K, Mazo A, Canaani E.; Structure and expression pattern of human ALR, a novel gene with strong homology to ALL-1 involved in acute leukemia and to Drosophila trithorax.; Oncogene. 1997 Jul 31;15(5):549-60.; Pubmed 9247308

Target Info

• Gene Symbol: KMT2D
• Gene Full Name: lysine methyltransferase 2D
• Alias Symbols: AAD10;ALL1-related protein;ALR;CAGL114;histone-lysine N-methyltransferase 2D;histone-lysine N-methyltransferase MLL2;KABUK1;Kabuki make-up syndrome;Kabuki mental retardation syndrome;KMS;lysine (K)-specific methyltransferase 2D;lysine N-methyltransferase 2D;MLL2;MLL4;myeloid/lymphoid or mixed-lineage leukemia 2;TNRC21;trinucleotide repeat containing 21;truncated lysine methyltransferase 2D.
• NCBI Gene Id: 8085
• Protein Name: Histone-lysine N-methyltransferase MLL2
• Uniprot ID: O14686
• Protein Accession #: NP_003473.3
• Molecular Weight: 593