Catalog No: OKEH03401
Size:96T
Price: $600.00
SKU
OKEH03401
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH03401
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MLKL ELISA Kit (Human) (OKEH03401) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MLKL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for MLKL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MLKL captured in well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.8% (n = 20)
Mean Inter-assay CV%: <= 7.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-MLKL Microplate96 Wells (12 x 8 Well strips)
MLKL Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated MLKL Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.087 ng/mL
SpecificityNatural and recombinant Human Mixed lineage kinase domain-like protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolMLKL
Gene Full Namemixed lineage kinase domain like pseudokinase
Alias SymbolshMLKL;mixed lineage kinase domain-like protein.
NCBI Gene Id197259
Protein NameQ8NB16
Description of TargetMixed lineage kinase domain-like protein
Uniprot IDhttps://www.uniprot.org/uniprot/Q8NB16
Protein Accession #NP_001135969.1
Nucleotide Accession #NM_001142497.2
Protein Size (# AA)471
Molecular Weight54 kDa
Write Your Own Review
You're reviewing:MLKL ELISA Kit (Human) (OKEH03401)
Your Rating
We found other products you might like!