MKI67 Antibody (OAAF01852)

Catalog No: OAAF01852 (Formerly GWB-ASD123)

Size:100 ug

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Ki67 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of COS7 cells, using Ki67 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Ki67.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: Ki67 antibody detects endogenous levels of total ki67protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:20000
• Reference: Mitch Dowsett, J Natl Cancer Inst, Jan 2007; 99: 167 - 170.
  Phyllis A. Gimotty, J. Clin. Oncol, Nov 2005; 23: 8048 - 8056.
  Tim J. Dudderidge, Clin. Cancer Res., Apr 2005; 11: 2510 - 2517.
  Scott F. Sieg, J. Immunol., Nov 2003; 171: 5208.
• Publications:

  Mesenchymal stem cells increase expression of heme oxygenase-1 leading to anti-inflammatory activity in treatment of acute liver failure. Stem Cell Res Ther. 8, 70 (2017). 28320485

Target Info

• Gene Symbol: MKI67
• Gene Full Name: marker of proliferation Ki-67
• Alias Symbols: antigen identified by monoclonal antibody Ki-67;antigen Ki67;KIA;MIB-;MIB-1;Molecular Immunology Borstel antibody 1;PPP1R105;proliferation marker protein Ki-67;proliferation-related Ki-67 antigen;protein phosphatase 1, regulatory subunit 105.
• NCBI Gene Id: 4288
• Protein Name: Proliferation marker protein Ki-67
• Description of Target: Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
• Uniprot ID: P46013
• Protein Accession #: NP_001139438.1
• Molecular Weight: 358 kDa