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- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for MIF Antibody (OAEB01140) |
|---|
| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | WB |
| Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
| Immunogen | MIF |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Human, Rat, Mouse |
| Peptide Sequence | C-NAANVGWNNSTFA |
| Concentration | 0.5 mg/ml |
| Application Info | P-ELISA: Antibody detection limit dilution 1:128000 WB: Approx 13kDa band observed in Human Lymph Node, and Thymus lysates and similar bands are observed in lysates of cell lines Daudi and Jurkat (calculated MW of 12.5kDa according to NP_002406.1). Recommended concentration: 0.01-0.03 ug/ml. Primary incubation was 1 hour. IHC: Paraffin embedded Human Thymus. Recommended concentration: 5 ug/ml. EIA: Sandwich-type ELISA with increasing amount of recombinant MIF captured by a rabbit antibody. Recommended reporter concentration: 1.5-3 ug/ml |
| Reference | * Church TS, Willis MS, Priest EL, Lamonte MJ, Earnest CP, Wilkinson WJ, Wilson DA, Giroir BP.; Obesity, macrophage migration inhibitory factor, and weight loss.; Int J Obes Relat Metab Disord. 2005 Mar 29; [Epub ahead of print]; Pubmed 15795748 |
| Gene Symbol | MIF |
|---|---|
| Gene Full Name | macrophage migration inhibitory factor |
| Alias Symbols | epididymis secretory sperm binding protein;GIF;GLIF;L-dopachrome isomerase;L-dopachrome tautomerase;macrophage migration inhibitory factor;macrophage migration inhibitory factor (glycosylation-inhibiting factor);MMIF;phenylpyruvate tautomerase. |
| NCBI Gene Id | 4282 |
| Protein Name | Macrophage migration inhibitory factor |
| Uniprot ID | P14174 |
| Protein Accession # | NP_002406.1 |
| Molecular Weight | 12.5 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MIF Antibody (OAEB01140)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "MIF Antibody (OAEB01140)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "MIF Antibody (OAEB01140)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MIF Antibody (OAEB01140)"?
This target may also be called "epididymis secretory sperm binding protein;GIF;GLIF;L-dopachrome isomerase;L-dopachrome tautomerase;macrophage migration inhibitory factor;macrophage migration inhibitory factor (glycosylation-inhibiting factor);MMIF;phenylpyruvate tautomerase." in publications.
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What is the shipping cost for "MIF Antibody (OAEB01140)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MIF Antibody (OAEB01140)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MIF Antibody (OAEB01140)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "12.5".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MIF Antibody (OAEB01140)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MIF"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MIF"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MIF"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MIF"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MIF"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MIF"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
