MID1IP1 Antibody (OAEB01078)

Catalog No: OAEB01078 (Formerly GWB-EV0966)

Size:100UG

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MID1IP1 with C-terminal MYC tag probed with OAEB01078 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with OAEB01078 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Epitope Info: Internal region
  Immunogen: Peptide with sequence C-HTVLSKLTRK, from the internal region of the protein sequence according to NP_067065.1.
  Reactivity Info: Tested: Human; Expected: Human, Mouse, Rat, Dog, Cow
  Product Info: Reported variants represent identical protein: NP_001092261.1, NP_067065.1, NP_001092260.1
• Reconstitution and Storage: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
  Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: MID1IP1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Guinea Pig: 100%; Mouse: 100%; Rat: 100%
• Peptide Sequence: C-HTVLSKLTRK
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:8000.
  Western Blot: In transfected HEK293 transiently expressing full-length Human MID1IP1 (myc and DYKDDDDK tagged), a band of approx. 28kDa was observed. No bands were
  observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1ug/ml.
• Reference: Berti C, Fontanella B, Ferrentino R, Meroni G, ; Mig12, a novel Opitz syndrome gene product partner, is expressed in the embryonic ventral midline and co-operates with Mid1 to bundle and stabilize microtubules.; BMC cell biology 2004 Feb 5 : 9.; PMID: 15070402

Target Info

• Gene Symbol: Mid1ip1|MID1IP1
• Gene Full Name: MID1 interacting protein 1|Mid1 interacting protein 1 (gastrulation specific G12-like (zebrafish))
• Alias Symbols: 3110038L01Rik;G12-like;gastrulation specific G12 homolog;gastrulation-specific G12-like protein;MID1 interacting G12-like protein;MID1 interacting protein 1 (gastrulation specific G12 homolog);MID1 interacting protein 1 (gastrulation specific G12-like);mid1-interacting G12-like protein;mid1-interacting protein 1;Mig1;MIG12;protein STRAIT11499 homolog;S14R;slap;spot 14-like androgen-inducible protein;spot 14-R;spot 14-related protein;STRAIT11499;THRSPL.
• NCBI Gene Id: 404280|58526|68041
• Protein Name: Mid1-interacting protein 1
• Uniprot ID: Q9NPA3
• Protein Accession #: NP_067065.1
• Molecular Weight: 20.2