MIA2 ELISA Kit (Mouse) (OKEH03635)

Catalog No: OKEH03635

Size:96T

Mouse MIA2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology MIA2 ELISA Kit (Mouse) (OKEH03635) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Mia2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Mia2 and incubated. The Mia2 found in the sample or standards competes with the biotinylated Mia2 for limited binding sites on the immobilized anti-human Mia2 antibody. Excess unbound biotinylated Mia2 and sample or standard Mia2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Mia2 captured in the well and inversely proportional to the amount of Mia2 which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 8.3% (n = 20)
  Mean Inter-assay CV%: <= 10.2% (n = 20)
• ELISA Kit Component:
  • Anti-Mia2 Microplate: 96 Wells (12 x 8 Well strips)
  • Mia2 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Mia2-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.178 ng/mL
• Specificity: Natural and recombinant Mouse Melanoma inhibitory activity protein 2
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Mia2
• Gene Full Name: MIA SH3 domain ER export factor 2
• Alias Symbols: Ctage;CTAGE family member 5 ER export factor;CTAGE family, member 5;Ctage5;cutaneous T-cell lymphoma-associated antigen 5 homolog;D12Bwg0579e;MEA;Mea6;melanoma inhibitory activity 2;melanoma inhibitory activity protein 2;meningioma expressed antigen 6 (coiled-coil proline-rich);Mge;Mgea;Mgea6;protein cTAGE-5.
• NCBI Gene Id: 338320
• Protein Name: Q91ZV0
• Description of Target: Melanoma inhibitory activity protein 2
• Uniprot ID: https://www.uniprot.org/uniprot/Q91ZV0
• Protein Accession #: NP_796295.1
• Nucleotide Accession #: NM_177321.2
• Protein Size (# AA): 517
• Molecular Weight: 58 kDa