MGEA5 ELISA Kit (Mouse) (OKCD01105)

Catalog No: OKCD01105 (Formerly GWB-KBBXY6)

Size:96WELLS

Mouse Mgea5 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	90-101%	92-101%	88-96%	81-91%
  EDTA plasma(n=5)	88-101%	86-93%	87-98%	78-92%
  heparin plasma(n=5)	78-91%	78-103%	80-101%	80-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Meningioma Expressed Antigen 5 (MGEA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Meningioma Expressed Antigen 5 (MGEA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Meningioma Expressed Antigen 5 (MGEA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Meningioma Expressed Antigen 5 (MGEA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	90-103	96
  EDTA plasma(n=5)	84-92	87
  heparin plasma(n=5)	83-104	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Meningioma Expressed Antigen 5 (MGEA5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Meningioma Expressed Antigen 5 (MGEA5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Mgea5 Microplate: 96 Wells (12 x 8 Well Strips)
  • Mgea5 Lyophilized Standard: 2 x 200 ng
  • 100X Biotinylated Mgea5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 1.28 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of N-Acetyl Beta-D-Glucosaminidase (NAGase). No significant cross-reactivity or interference between N-Acetyl Beta-D-Glucosaminidase (NAGase) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Oga
• Gene Full Name: O-GlcNAcase
• Alias Symbols: beta-hexosaminidase;beta-N-acetylhexosaminidase;bifunctional protein NCOAT;Hy5;meningioma expressed antigen 5 (hyaluronidase);meningioma-expressed antigen 5;Mgea;Mgea5;N-acetyl-beta-D-glucosaminidase;N-acetyl-beta-glucosaminidase;Ncoat;nuclear cytoplasmic O-GlcNAcase and acetyltransferase;protein O-GlcNAcase.
• NCBI Gene Id: 76055
• Protein Name: Protein O-GlcNAcase
• Description of Target: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:16517082). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.
• Uniprot ID: Q9EQQ9
• Protein Accession #: NP_001135906
• Nucleotide Accession #: NM_001142434.1
• Protein Size (# AA): 916