MGAT1 Antibody (OAEB01077)

Catalog No: OAEB01077 (Formerly GWB-EV0967)

Size:100UG

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MGAT1 with C-terminal MYC tag probed with OAEB01077 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with OAEB01077 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Epitope Info: Internal region
  Immunogen: Peptide with sequence C-QVEKVRTNDR, from the internal region of the protein sequence according to NP_002397.2.
  Reactivity Info: Tested: Human; Expected: Human, Mouse, Rat, Dog, Cow
  Product Info: Reported variants represent identical protein: NP_002397.2, NP_001108090.1, NP_001108091.1, NP_001108092.1, NP_001108089.1.
• Reconstitution and Storage: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
  Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: MGAT1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: C-QVEKVRTNDR
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:8000.
  Western Blot: In transfected HEK293 transiently expressing full-length Human MGAT1 (myc and DYKDDDDK tagged), a band of approx. 55kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1ug/ml.
• Reference: Bousman CA, Glatt SJ, Everall IP, Tsuang MT, ; Genetic association studies of methamphetamine use disorders: A systematic review and synthesis.; American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics 2009 Dec 150B (8): 1025-49.; PMID: 19219857

Target Info

• Gene Symbol: Mgat1|MGAT1
• Gene Full Name: alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase|mannoside acetylglucosaminyltransferase 1
• Alias Symbols: alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase;glcNAc-T I;GLCNAC-TI;GLCT1;GLYT1;GNT-1;GnTI;GNT-I;mannoside acetylglucosaminyltransferase 1;mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase;MGAT;Mgat-;Mgat-1;MGAT1 gene for N-acetylglucosaminyltransferase;N-acetylglucosaminyltransferase I;N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I.
• NCBI Gene Id: 17308|4245|81519
• Protein Name: Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
• Uniprot ID: P26572
• Protein Accession #: NP_002397.2
• Molecular Weight: 50.9