MGAM ELISA Kit (Human) (OKCD00655)

Catalog No: OKCD00655 (Formerly GWB-KBBLX5)

Size:96WELLS

Human MGAM ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Maltase-glucoamylase (MGAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Maltase-glucoamylase (MGAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Maltase-glucoamylase (MGAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Maltase-glucoamylase (MGAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Maltase-glucoamylase (MGAM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Maltase-glucoamylase (MGAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MGAM Microplate: 96 Wells (12 x 8 Well Strips)
  • MGAM Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated MGAM Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.54 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Maltase Glucoamylase, Intestinal (MGA). No significant cross-reactivity or interference between Maltase Glucoamylase, Intestinal (MGA) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MGAM
• Gene Full Name: maltase-glucoamylase
• Alias Symbols: brush border hydrolase;maltase-glucoamylase (alpha-glucosidase);maltase-glucoamylase, intestinal;MG;MGA.
• NCBI Gene Id: 8972
• Protein Name: Maltase-glucoamylase, intestinal
• Description of Target: May serve as an alternate pathway for starch digestion when luminal alpha-amylase activity is reduced because of immaturity or malnutrition. May play a unique role in the digestion of malted dietary oligosaccharides used in food manufacturing.
• Uniprot ID: O43451
• Protein Accession #: NP_004659
• Nucleotide Accession #: NM_004668.2
• Protein Size (# AA): 1857