Catalog No: OKCD00342 (Formerly GWB-KBBLC3)
Size:96WELLS
Price: $675.00
SKU
OKCD00342
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Datasheets/ManualsPrintable datasheet for MFN1 ELISA Kit (Human) (OKCD00342)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitofusin 1 (MFN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitofusin 1 (MFN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitofusin 1 (MFN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mitofusin 1 (MFN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitofusin 1 (MFN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitofusin 1 (MFN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MFN1 Microplate96 Wells (12 x 8 Well Strips)
MFN1 Lyophilized Standard2 x 10 ng
100X Biotinylated MFN1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mitofusin 1 (MFN1). No significant cross-reactivity or interference between Mitofusin 1 (MFN1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMFN1
Gene Full Namemitofusin 1
Alias Symbolsfzo homolog;hfzo1;hfzo2;mitochondrial transmembrane GTPase Fzo-1;mitochondrial transmembrane GTPase FZO-2;mitofusin-1;putative transmembrane GTPase;transmembrane GTPase MFN1.
NCBI Gene Id55669
Protein NameMitofusin-1
Description of TargetMitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12475957, PubMed:12759376, PubMed:27920125, PubMed:28114303). Membrane clustering requires GTPase activity (PubMed:27920125). It may involve a major rearrangement of the coiled coil domains (PubMed:27920125, PubMed:28114303). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:12475957, PubMed:12759376). Overexpression induces the formation of mitochondrial networks (in vitro) (PubMed:12759376). Has low GTPase activity (PubMed:27920125, PubMed:28114303).
Uniprot IDQ8IWA4
Protein Accession #NP_284941
Nucleotide Accession #NM_033540.2
Protein Size (# AA)741
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