MFGE8/Lactadherin ELISA Kit (Human) (OKBB00535)

Catalog No: OKBB00535

Size:96 Wells

Human MFGE8/Lactadherin ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human MFGE8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MFGE8 has been precoated onto 96-well plates. Standards(NS0, L24-C387) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MFGE8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human MFGE8 amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	669	1295	2234	724	1443	2458
  SD	42.82	58.3	116.2	56.5	86.58	135.2
  CV%	6.4	4.5	5.2	7.8	6	5.5

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human Lactadherin Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human Lactadherin Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human Lactadherin Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human MFGE8
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: L24-C387
• Description: For quantitative detection of human MFGE8 in cell culture supernatants, serum, plasma(heparin, EDTA), saliva, urine and human milk.

Target Info

• Gene Symbol: MFGE8
• Gene Full Name: milk fat globule-EGF factor 8 protein
• Alias Symbols: BA46, Breast epithelial antigen BA46, Contains Medin, EDIL1, HMFG, hP47, HsT19888, Lactadherin, Lactadherin [Precursor], Medin, MFG E8, MFG-E8, MFGE8, MFGM, MFGM_HUMAN, Milk fat globule EGF factor 8, Milk fat globule EGF factor 8 protein, Milk fat globule-EGF factor 8, O acetyl disialoganglioside synthase, OAcGD3S, SED1, SPAG10, Sperm associated antigen 10, Sperm surface protein hP47
• NCBI Gene Id: 4240
• Protein Name: Lactadherin
• Description of Target: MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.
• Uniprot ID: Q08431
• Protein Accession #: NP_001108086.1
• Nucleotide Accession #: NM_001114614.2