Catalog No: OKEH00577
Size:96T
Price: $625.00
SKU
OKEH00577
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology METRNL ELISA Kit (Rat) (OKEH00577) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rat METRNL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Rat METRNL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rat METRNL captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit Recovery<= 6.3%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
METRNL Microplate96 Wells (12 x 8 Well strips)
METRNL Lyophilized Standard2
100X Biotinylated METRNL Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity41.6 pg/mL
SpecificityNatural and recombinant Rat Meteorin-like protein
Publications

Effects of electrical stimulation-induced resistance exercise training on white and brown adipose tissues and plasma meteorin-like concentration in rats. Physiol Rep. 8, e14540 (2020). 32812347

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolMetrnl
Gene Full Namemeteorin-like, glial cell differentiation regulator
Alias Symbolsmeteorin, glial cell differentiation regulator-like;meteorin-like protein;subfatin.
NCBI Gene Id316842
Protein NameQ5RJL6
Description of TargetMeteorin-like protein
Uniprot IDhttps://www.uniprot.org/uniprot/Q5RJL6
Protein Accession #NP_001014126.1
Nucleotide Accession #NM_001014104.1
Protein Size (# AA)311
Molecular Weight34 kDa
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