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Datasheets/Manuals | Printable datasheet for MER/SKY Antibody (Phospho-Tyr749/681) (OAAF00456) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Additional Information | Modification Sites: H: Y749/681, M: Y744/671, R: Y744:671 |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human MER/SKY around the phosphorylation site of Tyr749/681. |
Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
Concentration | 1 mg/ml |
Target Post-Translational Modification | Phospho |
Specificity | MER/SKY (Phospho-Tyr749/681) antibody detects endogenous levels of MER/SKY only when phosphorylated at tyrosine 749/681. |
Intended Use | The product is for research use only, not for use in diagnostic or therapeutic procedures. |
Application Info | WB: 1:500~1000 IHC: 1:50~100 ELISA: 1:10000 Optimal dilutions should be determined by the end user. |
Publications | Normalization of TAM post-receptor signaling reveals a cell invasive signature for Axl tyrosine kinase. Cell Commun. Signal. 14, 19 (2016). 27595981 |
Gene Symbol | MERTK|TYRO3 |
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Gene Full Name | MER proto-oncogene, tyrosine kinase|TYRO3 protein tyrosine kinase |
Alias Symbols | BYK;c-Eyk;c-mer;c-mer proto-oncogene tyrosine kinase;Dtk;Etk-2;MER;MER receptor tyrosine kinase;proto-oncogene c-Mer;receptor tyrosine kinase MerTK;Rek;RP38;RSE;Sky;STK kinase;Tif;Tyro12;tyrosine-protein kinase byk;tyrosine-protein kinase DTK;tyrosine-protein kinase Mer;tyrosine-protein kinase receptor TYRO3;tyrosine-protein kinase RSE;tyrosine-protein kinase SKY;tyrosine-protein kinase TIF. |
NCBI Gene Id | 10461|7301 |
Protein Name | Tyrosine-protein kinase Mer|Tyrosine-protein kinase receptor TYRO3 |
Description of Target | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.|Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. |
Uniprot ID | Q06418|Q12866 |
Protein Accession # | NP_006284.2 |
Molecular Weight | 110 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MER/SKY Antibody (Phospho-Tyr749/681) (OAAF00456)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "MER/SKY Antibody (Phospho-Tyr749/681) (OAAF00456)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "MER/SKY Antibody (Phospho-Tyr749/681) (OAAF00456)" provided in?
This item is provided in "Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MER/SKY Antibody (Phospho-Tyr749/681) (OAAF00456)"?
This target may also be called "BYK;c-Eyk;c-mer;c-mer proto-oncogene tyrosine kinase;Dtk;Etk-2;MER;MER receptor tyrosine kinase;proto-oncogene c-Mer;receptor tyrosine kinase MerTK;Rek;RP38;RSE;Sky;STK kinase;Tif;Tyro12;tyrosine-protein kinase byk;tyrosine-protein kinase DTK;tyrosine-protein kinase Mer;tyrosine-protein kinase receptor TYRO3;tyrosine-protein kinase RSE;tyrosine-protein kinase SKY;tyrosine-protein kinase TIF." in publications.
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What is the shipping cost for "MER/SKY Antibody (Phospho-Tyr749/681) (OAAF00456)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MER/SKY Antibody (Phospho-Tyr749/681) (OAAF00456)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MER/SKY Antibody (Phospho-Tyr749/681) (OAAF00456)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "110 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MER/SKY Antibody (Phospho-Tyr749/681) (OAAF00456)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MERTK|TYRO3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MERTK|TYRO3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MERTK|TYRO3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MERTK|TYRO3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MERTK|TYRO3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MERTK|TYRO3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.