Mer ELISA Kit (Human) (OKBB00329)

Catalog No: OKBB00329

Size:96 Tests

Human MERTK ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human Mer ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Mer has been precoated onto 96-well plates. Standards(NS0, R26-A499) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Mer is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Mer amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	1124	3287	5853	1374	3467	6124
  St. Dev.	58.45	210.4	280.9	92.1	249.6	306.2
  %CV	5.20	6.40	4.79	6.70	7.19	5

• ELISA Kit Component:
  • Lyophilized recombinant mouse C-MET standard: 10 ng/tube x 2
  • Anti-mouse C-MET Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-mouse C-MET antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 0.156ng/ml-10ng/ml
  Principle: Aviva’s human Mer ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Mer specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Mer amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before using.
  Background: Proto-oncogene tyrosine-protein kinase MER is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Its gene is mapped to chromosome 2q14.1. Mer encodes a 984-amino acid protein with a calculated molecular mass of 109 kD. It is expressed in numerous neoplastic B- and T-cell lines. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP)
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, cell lysates, tissue homogenates and serum
• Sensitivity: <10 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Graham DK, Dawson TL, Mullaney DL, Snodgrass HR, Earp HS (Oct 1994). "Cloning and mRNA expression analysis of
  a novel human proto-oncogene, c-mer". Cell Growth Differ 5 (6): 647–57. PMID 8086340.
  2. Weier HU, Fung J, Lersch RA (Jun 1999). "Assignment of proto-oncogene MERTK (a.k.a. c-mer) to human
  chromosome 2q14.1 by in situ hybridization". Cytogenetic Cell Genet 84 (1–2): 91–2. doi:10.1159/000015223. PMID
  10343112.
• Specificity: Natural and recombinant human Mer
• Immunogen: Expression system for standard: NS0; Immunogen sequence: R26-A499
• Description: For quantitative detection of human Mer in cell culture supernatants, cell lysates and serum.

Target Info

• Gene Symbol: MERTK
• Gene Full Name: c-mer proto-oncogene tyrosine kinase
• Alias Symbols: C mer, c mer proto oncogene tyrosine kinase, MER receptor tyrosine kinase, Mertk, MERTK_HUMAN, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK, RP38, STK kinase, Tyrosine-protein kinase Mer
• NCBI Gene Id: 10461
• Protein Name: Tyrosine-protein kinase Mer
• Description of Target: Proto-oncogene tyrosine-protein kinase MER is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Its gene is mapped to chromosome 2q14.1. Mer encodes a 984-amino acid protein with a calculated molecular mass of 109 kD. It is expressed in numerous neoplastic B- and T-cell lines. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP)
• Uniprot ID: Q12866
• Protein Accession #: NP_006334.2
• Nucleotide Accession #: NM_006343.2