Catalog No: OKCD02722
Size:96WELLS
Price: $700.00
SKU
OKCD02722
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Datasheets/ManualsPrintable datasheet for MELK ELISA kit (Mouse)
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Maternal Embryonic Leucine Zipper Kinase (MELK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Maternal Embryonic Leucine Zipper Kinase (MELK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Maternal Embryonic Leucine Zipper Kinase (MELK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Maternal Embryonic Leucine Zipper Kinase (MELK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Maternal Embryonic Leucine Zipper Kinase (MELK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Maternal Embryonic Leucine Zipper Kinase (MELK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MELK Microplate96 Wells (12 x 8 Well strips)
MELK Lyophilized Standard2
100X Biotinylated MELK Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.057 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Maternal Embryonic Leucine Zipper Kinase (MELK). No significant cross-reactivity or interference between Maternal Embryonic Leucine Zipper Kinase (MELK) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolMELK
Gene Full Namematernal embryonic leucine zipper kinase
Alias SymbolsMPK3, MPK38, AI327312, mKIAA0175
NCBI Gene Id17279
Protein NameMaternal embryonic leucine zipper kinase
Description of TargetSerine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
Uniprot IDQ61846
Protein Accession #NP_034920.2
Nucleotide Accession #NM_010790.2
Protein Size (# AA)643
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