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Now Offering Over 85,203 Antibodies & 34,301 Antigens!

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0.1 mg
$375.00
In Stock

MED28 Antibody (OAPB00724)

The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED28, also known as Magicin. MED28 is expressed in many cell lines and tissues. It has been shown that a down-regulation of MED28 expression in NIH3T3 cells results in a significant induction of several genes associated with smooth muscle cell differentiation and conversely its over-expression represses expression of SMC genes. MED28 can also form a ternary complex with Grb2 and Merlin, the neurofibromatosis 2 tumor suppressor protein, indicating that MED28 may play a role in Merlin's tumor suppressive activity. MED28 has also been recently identified as an HIV dependency factor (HDF), suggesting that MED28 may be an important drug target in HIV treatment. At least two isoforms of MED28 are known to exist.
Description of Target:
Mediator of RNA polymerase II transcription subunit 28
Gene Symbol:
MED28
Alias Symbols:
MED28; Mediator of RNA polymerase II transcription subunit 28; magicin; EG1
Host:
Rabbit
Clonality:
Polyclonal
Application:
ELISA, WB, IHC, IF
Immunogen:
The immunogen for anti-MED28 antibody: antibody was raised against a 17 amino acid peptide from near the center of human MED28.
Purification:
Affinity chromatography purified via peptide column
Predicted Homology Based on Immunogen Sequence:
Human, Mouse
Species Reactivity:
Human; Mouse
Datasheets / Downloads:
Printable datasheet for
anti-MED28 antibody
- OAPB00724
Applications Info:
MED28 antibody can be used for detection of MED28 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
POSITIVE CONTROL: 1) Cat. No. OCPB00143 - Human Brain Tissue Lysate
Blocking Peptide:
For anti-MED28 antibody is Catalog # OEPB02841
:::
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Key Reference:
Sato S, Tomomori-Sato C, Parmely TJ, et al. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Mol. Cell2004; 14:685-91.;Beyer KS, Beauchamp RL, Lee MF, et al. Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation. J. Biol. Chem.2007; 282:32152-7.;Wiederhold T, Lee MF, James M, et al. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Oncogene2004; 23:8815-25.;Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.
Reconstitution and Storage:
MED28 antibody can be stored at 4C for three months and -20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
 
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