Catalog No: OAPB00724
Size:100UG
Price: $461.00
SKU
OAPB00724
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for MED28 Antibody (OAPB00724) (OAPB00724)
Product Info
Predicted Species ReactivityBovine|Human|Mouse|Rat
Product FormatLiquid. MED28 Antibody is supplied in PBS containing 0.02% sodium azide.
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
ApplicationEnzyme-linked immunosorbent assay|Immunohistochemistry-Paraffin|Western blot
::Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Reconstitution and StorageMED28 antibody can be stored at 4C for three months and -20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ImmunogenMED28 antibody was raised against a 17 amino acid synthetic peptide from near the center of human MED28. The immunogen is located within amino acids 50 - 100 of MED28.
PurificationAffinity Purified
Predicted Homology Based on Immunogen SequenceHuman; Mouse
Concentration1 mg/ml
Blocking PeptideFor anti-MED28 antibody is Catalog # OEPB02841
Application InfoMED28 antibody can be used for detection of MED28 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

POSITIVE CONTROL: 1) Cat. No. OCPB00143 - Human Brain Tissue Lysate
ReferenceSato S, Tomomori-Sato C, Parmely TJ, et al. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Mol. Cell2004; 14:685-91.;Beyer KS, Beauchamp RL, Lee MF, et al. Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation. J. Biol. Chem.2007; 282:32152-7.;Wiederhold T, Lee MF, James M, et al. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Oncogene2004; 23:8815-25.;Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.
Gene SymbolMED28
Gene Full Namemediator complex subunit 28
Alias Symbols1500003D12Rik;EG1;endothelial-derived gene 1;endothelial-derived protein 1;magicin;Mediator complex subunit 28;mediator of RNA polymerase II transcription subunit 28;mediator of RNA polymerase II transcription, subunit 28 homolog;merlin and Grb2-interacting cytoskeletal protein;tumor angiogenesis marker EG-1.
NCBI Gene Id80306
Protein NameMediator of RNA polymerase II transcription subunit 28|mediator of RNA polymerase II transcription subunit 28 [Homo sapiens]
Description of TargetComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.
Uniprot IDQ9H204
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_079481
  1. What is the species homology for "MED28 Antibody (OAPB00724)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Human|Mouse|Rat".

  2. How long will it take to receive "MED28 Antibody (OAPB00724)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "MED28 Antibody (OAPB00724)" provided in?

    This item is provided in "Liquid. MED28 Antibody is supplied in PBS containing 0.02% sodium azide.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "MED28 Antibody (OAPB00724)"?

    This target may also be called "1500003D12Rik;EG1;endothelial-derived gene 1;endothelial-derived protein 1;magicin;Mediator complex subunit 28;mediator of RNA polymerase II transcription subunit 28;mediator of RNA polymerase II transcription, subunit 28 homolog;merlin and Grb2-interacting cytoskeletal protein;tumor angiogenesis marker EG-1." in publications.

  5. What is the shipping cost for "MED28 Antibody (OAPB00724)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "MED28 Antibody (OAPB00724)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "MED28 Antibody (OAPB00724)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "MED28 Antibody (OAPB00724)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "MED28"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "MED28"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "MED28"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "MED28"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "MED28"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "MED28"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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