MED24 Antibody (OAAF02055)

Catalog No: OAAF02055 (Formerly GWB-ASD326)

Size:100 ug

Western blot analysis of extracts from 293 cells, using MED24 Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HuvEc cells, using MED24 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MED24.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: MED24 antibody detects endogenous levels of total MED24 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IF: 1:100~1:500; ELISA: 1:20000
• Reference: Yuan C.-X., Proc. Natl. Acad. Sci. U.S.A. 95:7939-7944(1998).
  Rachez C., Genes Dev. 12:1787-1800(1998).
  Nagase T., DNA Res. 2:167-174(1995).

Target Info

• Gene Symbol: MED24
• Gene Full Name: mediator complex subunit 24
• Alias Symbols: activator-recruited cofactor 100 kDa component;ARC100;cofactor required for Sp1 transcriptional activation subunit 4;cofactor required for Sp1 transcriptional activation, subunit 4, 100kDa;CRSP complex subunit 4;CRSP100;CRSP4;DRIP100;MED5;Mediator complex subunit 24;mediator of RNA polymerase II transcription subunit 24;mediator of RNA polymerase II transcription, subunit 24 homolog;THRAP4;thyroid hormone receptor-associated protein 4;thyroid hormone receptor-associated protein complex 100 kDa component;TRAP100;vitamin D3 receptor-interacting protein complex 100 kDa component;vitamin D3 receptor-interacting protein complex component DRIP100.
• NCBI Gene Id: 9862
• Protein Name: Mediator of RNA polymerase II transcription subunit 24
• Description of Target: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
• Uniprot ID: O75448
• Protein Accession #: NP_001072986.1
• Molecular Weight: 110 kDa