MED23 Antibody (OAAF02744)

Catalog No: OAAF02744 (Formerly GWB-ASD943)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MED23 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of A549 cells, using MED23 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MED23.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: MED23 antibody detects endogenous levels of total MED23 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:1000
• Reference: Wiemann S., Genome Res. 11:422-435(2001).
  Mungall A.J., Nature 425:805-811(2003).
  Akoulitchev S., Nature 407:102-106(2000).

Target Info

• Gene Symbol: MED23
• Gene Full Name: mediator complex subunit 23
• Alias Symbols: 130 kDa transcriptional co-activator;133 kDa transcriptional co-activator;activator-recruited cofactor 130 kDa component;ARC130;cofactor required for Sp1 transcriptional activation subunit 3;CRSP130;CRSP133;CRSP3;DRIP130;Mediator complex subunit 23;mediator of RNA polymerase II transcription subunit 23;mental retardation, non-syndromic, autosomal recessive, 18;MRT18;Protein sur-2 homolog;SUR2;SUR-2;Transcriptional coactivator CRSP130;vitamin D3 receptor interacting protein;Vitamin D3 receptor-interacting protein complex 130 kDa component.
• NCBI Gene Id: 9439
• Protein Name: Mediator of RNA polymerase II transcription subunit 23
• Description of Target: Required for transcriptional activation subsequent to the assembly of the pre-initiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.
• Uniprot ID: Q9ULK4
• Protein Accession #: NP_001257450.1
• Molecular Weight: 156 kDa