MED12 Antibody (OAAF02597)

Catalog No: OAAF02597

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using MED12 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MED12.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: MED12 antibody detects endogenous levels of total MED12 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:5000
• Reference: Ito M., Mol. Cell 3:361-370(1999).
  Nagase T., DNA Res. 3:17-24(1996).
  Philibert R.A., Mol. Psychiatry 3:303-309(1998).

Target Info

• Gene Symbol: MED12
• Gene Full Name: mediator complex subunit 12
• Alias Symbols: activator-recruited cofactor 240 kDa component;ARC240;CAG repeat protein 45;CAGH45;FGS1;HOPA;human opposite paired;Kohtalo homolog;Kto;MED12S;Mediator complex subunit 12;mediator of RNA polymerase II transcription subunit 12;mediator of RNA polymerase II transcription, subunit 12 homolog;OHDOX;OKS;OPA1;OPA-containing protein;putative mediator subunit 12;thyroid hormone receptor-associated protein complex 230 kDa component;thyroid hormone receptor-associated protein, 230 kDa subunit;TNRC11;TRAP230;trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit);trinucleotide repeat-containing gene 11 protein.
• NCBI Gene Id: 9968
• Protein Name: Mediator of RNA polymerase II transcription subunit 12
• Description of Target: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
• Uniprot ID: Q93074
• Protein Accession #: NP_005111.2
• Molecular Weight: 247 kDa