Catalog No: OAAF00099 (Formerly GWB-ASB171)
Size:100 ug
Price: $344.00
SKU
OAAF00099
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for MDM2 Antibody (Phospho-Ser166) (OAAF00099)
Product Info
Predicted Species ReactivityHuman|Monkey|Mouse
Product FormatLiquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ClonalityPolyclonal
HostRabbit
ApplicationEnzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Reconstitution and Storage-20°C
ImmunogenThe antiserum was produced against synthesized peptide derived from human MDM2 around the phosphorylation site of Ser166.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Application InfoWB 1:500~1000
IHC 1:50~100
IF 1:100~500
ELISA 1:5000
ReferenceM Zhou, Blood, Mar 1995; 85: 1608 - 1614.
Susan M. Mendrysa, Mol. Cell. Biol., Mar 2000; 20: 2023 - 2030.
M. Saeed Sheikh, Cancer Res., Jul 1993; 53: 3226 - 3228.
Jordan S. Fridman, Cancer Res., Sep 2003; 63: 5703 - 5706.
Gene SymbolMDM2
Gene Full NameMDM2 proto-oncogene
Alias SymbolsACTFS;Double minute 2 protein;double minute 2, human homolog of; p53-binding protein;E3 ubiquitin-protein ligase Mdm2;hdm2;HDMX;LSKB;MDM2 oncogene, E3 ubiquitin protein ligase;MDM2 proto-oncogene, E3 ubiquitin protein ligase;Mdm2, p53 E3 ubiquitin protein ligase homolog;Mdm2, transformed 3T3 cell double minute 2, p53 binding protein;oncoprotein Mdm2;p53-binding protein Mdm2;RING-type E3 ubiquitin transferase Mdm2.
NCBI Gene Id4193
Protein NameE3 ubiquitin-protein ligase Mdm2
Description of TargetE3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (PubMed:12821780, PubMed:15053880, PubMed:15195100, PubMed:15632057, PubMed:16337594, PubMed:17290220, PubMed:19098711, PubMed:19219073, PubMed:19837670, PubMed:19965871, PubMed:20173098, PubMed:20385133, PubMed:20858735, PubMed:22128911). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells (By similarity). Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis (By similarity).
Uniprot IDQ00987
Protein Accession #NP_002383.2
Molecular Weight55 kDa
  1. What is the species homology for "MDM2 Antibody (Phospho-Ser166) (OAAF00099)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Monkey|Mouse".

  2. How long will it take to receive "MDM2 Antibody (Phospho-Ser166) (OAAF00099)"?

    This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".

  3. What buffer format is "MDM2 Antibody (Phospho-Ser166) (OAAF00099)" provided in?

    This item is provided in "Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "MDM2 Antibody (Phospho-Ser166) (OAAF00099)"?

    This target may also be called "ACTFS;Double minute 2 protein;double minute 2, human homolog of; p53-binding protein;E3 ubiquitin-protein ligase Mdm2;hdm2;HDMX;LSKB;MDM2 oncogene, E3 ubiquitin protein ligase;MDM2 proto-oncogene, E3 ubiquitin protein ligase;Mdm2, p53 E3 ubiquitin protein ligase homolog;Mdm2, transformed 3T3 cell double minute 2, p53 binding protein;oncoprotein Mdm2;p53-binding protein Mdm2;RING-type E3 ubiquitin transferase Mdm2." in publications.

  5. What is the shipping cost for "MDM2 Antibody (Phospho-Ser166) (OAAF00099)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "MDM2 Antibody (Phospho-Ser166) (OAAF00099)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "MDM2 Antibody (Phospho-Ser166) (OAAF00099)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "55 kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "MDM2 Antibody (Phospho-Ser166) (OAAF00099)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "MDM2"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "MDM2"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "MDM2"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "MDM2"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "MDM2"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "MDM2"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

Write Your Own Review
You're reviewing:MDM2 Antibody (Phospho-Ser166) (OAAF00099)
Your Rating
We found other products you might like!