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- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for MDM2 Antibody (OASA03475) |
|---|
| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Human |
| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | SMP14 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunohistochemistry-Resin |
| Additional Information | Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line. Histology Positive Control Tissue: Breast carcinoma Carrier free: Yes |
| Reconstitution and Storage | -20°C |
| Immunogen | Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2. |
| Purification | Protein A Purified |
| Concentration | 1 mg/ml |
| Specificity | MDM2. Reacts with mouse. Antibody reactivity and working conditions may vary between species. |
| Application Info | IHC-P: 1/200 - 1/500. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA pH 8.0 is recommended for this purpose. |
| Reference | 1. Picksley, S.M. et al. (1994) Immunochemical analysis of the interaction of p53 with MDM2; - fine mapping of the MDM2 binding site on p53 using synthetic peptides. Oncogene 9: 2523-2529. 2. Martin, K. et al. (1995) Stimulation of E2F1/DP1 transcriptional activity by MDM2 oncoprotein. Nature 375: 691-694. 3. Gueven, N. et al. (2006) Defective p53 response and apoptosis associated with an ataxia-telangiectasia-like phenotype. |
| Gene Symbol | MDM2 |
|---|---|
| Alias Symbols | PERB11.2 |
| NCBI Gene Id | 4277 |
| Protein Name | E3 ubiquitin-protein ligase Mdm2 |
| Description of Target | Mouse anti MDM2 antibody, clone SMP14 recognizes the MDM2 protein, which forms a complex with p53 leading to p53 inhibition. MDM2 also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI. MDM2 is over expressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer. |
| Uniprot ID | Q00987 |
| Protein Accession # | NP_002383.2 |
| Protein Size (# AA) | 497 |
| Molecular Weight | 90 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MDM2 Antibody (OASA03475)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
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How long will it take to receive "MDM2 Antibody (OASA03475)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "MDM2 Antibody (OASA03475)" provided in?
This item is provided in "Liquid. Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MDM2 Antibody (OASA03475)"?
This target may also be called "PERB11.2" in publications.
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What is the shipping cost for "MDM2 Antibody (OASA03475)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MDM2 Antibody (OASA03475)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MDM2 Antibody (OASA03475)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "90 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MDM2 Antibody (OASA03475)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MDM2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MDM2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MDM2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MDM2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MDM2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MDM2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
