Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD02635
Size:96WELLS
Price: $725.00
SKU
OKCD02635
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for MDH1 ELISA Kit (Rat) (OKCD02635)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)91-105%90-98%95-102%81-95%
EDTA Plasma (n=5)83-92%78-88%78-103%91-104%
Heparin Plasma (n=5)97-105%99-105%81-90%99-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Malate Dehydrogenase 1 (MDH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Malate Dehydrogenase 1 (MDH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Malate Dehydrogenase 1 (MDH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Malate Dehydrogenase 1 (MDH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)89-10294
EDTA Plasma (n=5)97-105101
Heparin Plasma(n=5)88-9692
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Malate Dehydrogenase 1 (MDH1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Malate Dehydrogenase 1 (MDH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MDH1 Microplate96 Wells (12 x 8 Well strips)
MDH1 Lyophilized Standard2
100X Biotinylated MDH1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.072 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Malate Dehydrogenase 1 (MDH1). No significant cross-reactivity or interference between Malate Dehydrogenase 1 (MDH1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolMdh1
Gene Full Namemalate dehydrogenase 1
Alias Symbolscytosolic malate dehydrogenase;malate dehydrogenase 1, NAD (soluble);malate dehydrogenase, cytoplasmic;malate dehydrogenase, peroxisomal;malate dehydrogenase, soluble;Malate dehydrogenase-like enzyme;Mdhl;MDL1;Mor2.
NCBI Gene Id24551
Protein NameMalate dehydrogenase, cytoplasmic
Uniprot IDO88989
Protein Accession #NP_150238.1
Nucleotide Accession #NM_033235.2
Protein Size (# AA)334
Write Your Own Review
You're reviewing:MDH1 ELISA Kit (Rat) (OKCD02635)
Your Rating
We found other products you might like!