MDH1 ELISA Kit (Human) (OKCD02721)

Catalog No: OKCD02721

Size:96WELLS

Human MDH1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	90-98%	96-104%	98-105%	89-103%
  EDTA Plasma (n=5)	95-104%	84-101%	81-94%	84-92%
  Heparin Plasma (n=5)	90-99%	78-91%	93-105%	85-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Malate Dehydrogenase 1 (MDH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Malate Dehydrogenase 1 (MDH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Malate Dehydrogenase 1 (MDH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Malate Dehydrogenase 1 (MDH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95-103	99
  EDTA Plasma (n=5)	81-98	87
  Heparin Plasma(n=5)	97-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Malate Dehydrogenase 1 (MDH1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Malate Dehydrogenase 1 (MDH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MDH1 Microplate: 96 Wells (12 x 8 Well strips)
  • MDH1 Lyophilized Standard: 2
  • 100X Biotinylated MDH1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 35 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Malate Dehydrogenase 1 (MDH1). No significant cross-reactivity or interference between Malate Dehydrogenase 1 (MDH1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MDH1
• Gene Full Name: malate dehydrogenase 1
• Alias Symbols: cytosolic malate dehydrogenase;DEE88;diiodophenylpyruvate reductase;EIEE88;epididymis secretory protein Li 32;HEL-S-32;KAR;malate dehydrogenase 1, NAD (soluble);malate dehydrogenase, cytoplasmic;malate dehydrogenase, peroxisomal;MDHA;MDH-s;MGC:1375;MOR2;soluble malate dehydrogenase.
• NCBI Gene Id: 4190
• Protein Name: Malate dehydrogenase, cytoplasmic
• Description of Target: Malate dehydrogenase, cytoplasmic
• Uniprot ID: P40925
• Protein Accession #: NP_001186040.1
• Nucleotide Accession #: NM_001199111.1
• Protein Size (# AA): 334