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Datasheets/Manuals | Printable datasheet for anti-MCM4 (OASG04412) antibody |
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Predicted Species Reactivity | Human, Mouse, Monkey |
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Product Format | Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | WB, IHC |
Reconstitution and Storage | Store at -20C. Avoid repeated freeze/thaw cycles. |
Immunogen | Synthesized peptide derived from human MCM4 around the phosphorylation site of S54. Location: 30-110aa. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration | 1 mg/ml |
Target Post-Translational Modification | Phospho-Ser54 |
Specificity | Phospho-MCM4 (S54) Polyclonal Antibody detects endogenous levels of MCM4 protein only when phosphorylated at S54. |
Application Info | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; |
Gene Symbol | MCM4 |
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Gene Full Name | minichromosome maintenance complex component 4 |
Alias Symbols | NKCD, CDC21, CDC54, IMD54, NKGCD, hCdc21, P1-CDC21 |
NCBI Gene Id | 4173; 17217; |
Description of Target | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. |
Uniprot ID | P33991, P49717 |
Molecular Weight | 85 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MCM4 Antibody (Phospho-Ser54) (OASG04412)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Monkey".
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How long will it take to receive "MCM4 Antibody (Phospho-Ser54) (OASG04412)"?
This item is available "Domestic: within 2-3 weeks delivery | International: 2-3 weeks".
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What buffer format is "MCM4 Antibody (Phospho-Ser54) (OASG04412)" provided in?
This item is provided in "Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MCM4 Antibody (Phospho-Ser54) (OASG04412)"?
This target may also be called "NKCD, CDC21, CDC54, IMD54, NKGCD, hCdc21, P1-CDC21" in publications.
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What is the shipping cost for "MCM4 Antibody (Phospho-Ser54) (OASG04412)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MCM4 Antibody (Phospho-Ser54) (OASG04412)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MCM4 Antibody (Phospho-Ser54) (OASG04412)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "85 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MCM4 Antibody (Phospho-Ser54) (OASG04412)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MCM4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MCM4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MCM4"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MCM4"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MCM4"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MCM4"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.