MCM4 Antibody (OAAF01028)

Catalog No: OAAF01028 (Formerly GWB-ASC431)

Size:100 ug

Western blot analysis of extracts from NIH-3T3/K562 cells, using MCM4 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using MCM4 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MCM4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: MCM4 (Ab-54) antibody detects endogenous levels of total MCM4 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:20000
• Reference: Musahl C., Eur. J. Biochem. 230:1096-1101(1995).
  Livingston R.J., Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
  The MGC Project Team; Genome Res. 14:2121-2127(2004)

Target Info

• Gene Symbol: MCM4
• Gene Full Name: minichromosome maintenance complex component 4
• Alias Symbols: CDC21;CDC21 homolog;CDC54;DNA replication licensing factor MCM4;hCdc21;homolog of S. pombe cell devision cycle 21;IMD54;minichromosome maintenance deficient 4;NKCD;NKGCD;P1-CDC21.
• NCBI Gene Id: 4173
• Protein Name: DNA replication licensing factor MCM4
• Description of Target: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
• Uniprot ID: P33991
• Protein Accession #: NP_005905.2
• Molecular Weight: 96 kDa