MCL1 Antibody (OAEB01504)

Catalog No: OAEB01504 (Formerly GWB-5A1B12)

Size:100UG

OAEB01504 staining (1ug/ml) of Human Lung lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: MCL1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 83%
• Peptide Sequence: C-AGVGAGLAYLIR
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:2000.
  Western Blot: Approx 35kDa band observed in lysates of Human Lung and cell line K562 (calculated MW of 37.3kDa according to NP_068779.1). Recommended concentration: 1-3ug/ml.
• Reference: * Donaldson M, Antignani A, Milner J, Zhu N, Wood A, Cardwell-Miller L, Changpriroa CM, Jackson SH.; p47phox-deficient immune microenvironment signals dysregulate naive T-cell apoptosis.; Cell Death Differ. 2009 Jan;16(1):125-38.; Pubmed 18806761
  * Opferman JT, Letai A, Beard C, Sorcinelli MD, Ong CC, Korsmeyer SJ.; Development and maintenance of B and T lymphocytes requires antiapoptotic MCL-1.; Nature. 2003 Dec 11;426(6967):671-6.; Pubmed 14668867
  * Opferman JT, Letai A, Beard C, Sorcinelli MD, Ong CC, Korsmeyer SJ.; Development and maintenance of B and T lymphocytes requires antiapoptotic MCL-1.; Nature. 2003 Dec 11;426(6967):671-6.; Pubmed 14668867

Target Info

• Gene Symbol: Mcl1|MCL1
• Gene Full Name: MCL1 apoptosis regulator, BCL2 family member|myeloid cell leukemia sequence 1
• Alias Symbols: AW556805;BCL2 family apoptosis regulator;bcl2-L-3;BCL2L3;bcl-2-like protein 3;bcl-2-related protein EAT/mcl1;EAT;induced myeloid leukemia cell differentiation protein Mcl-1;induced myeloid leukemia cell differentiation protein Mcl-1 homolog;Mcl-;Mcl-1;MCL1, BCL2 family apoptosis regulator;mcl1/EAT;MCL1-ES;MCL1L;MCL1S;myeloid cell leukemia 1;myeloid cell leukemia ES;myeloid cell leukemia sequence 1 (BCL2-related);TM.
• NCBI Gene Id: 17210|4170
• Protein Name: Induced myeloid leukemia cell differentiation protein Mcl-1
• Uniprot ID: Q07820
• Protein Accession #: NP_068779.1
• Molecular Weight: 37.3; 21.2