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Catalog No: OKCD02716
Size:96WELLS
Price: $625.00
SKU
OKCD02716
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Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)92-99%97-105%98-105%78-95%
EDTA Plasma (n=5)87-103%98-105%92-101%93-105%
Heparin Plasma (n=5)94-101%88-96%85-101%92-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myelin Basic Protein (MBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myelin Basic Protein (MBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myelin Basic Protein (MBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myelin Basic Protein (MBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)79-10482
EDTA Plasma (n=5)82-8986
Heparin Plasma(n=5)78-9786
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myelin Basic Protein (MBP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myelin Basic Protein (MBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MBP Microplate96 Wells (12 x 8 Well strips)
MBP Lyophilized Standard2
100X Biotinylated MBP Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity5.9 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Myelin Basic Protein (MBP). No significant cross-reactivity or interference between Myelin Basic Protein (MBP) and analogues was observed.
Publications

Teruya, Shihono et al. Bordetella Dermonecrotic Toxin Is a Neurotropic Virulence Factor That Uses CaV3.1 as the Cell Surface Receptor. mBio. 2020 Mar-Apr 11(2): e03146-19. 32209694

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Description
Gene SymbolMbp
Gene Full Namemyelin basic protein
Alias SymbolsC76307;goll;Golli-Mbp;Hmb;Hmbpr;jv;jve;mld;myelin A1 protein;myelin basic protein;myelin basic protein transcript variant X5;myelin deficient;R75289;shi;shiverer.
NCBI Gene Id17196
Protein NameMyelin basic protein
Description of TargetThe classic group of MBP isoforms (isoform 4-isoform 13) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function.
Uniprot IDP04370
Protein Accession #NP_001020416.1
Nucleotide Accession #NM_001025245.1
Protein Size (# AA)250
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