Catalog No: OKCD02716
Size:96WELLS
Price: $625.00
SKU
OKCD02716
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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MBP ELISA Kit (Mouse) (OKCD02716)
Datasheets/Manuals | Printable datasheet for MBP ELISA Kit (Mouse) (OKCD02716) |
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COA Datasheet | ClickHereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myelin Basic Protein (MBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myelin Basic Protein (MBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myelin Basic Protein (MBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myelin Basic Protein (MBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myelin Basic Protein (MBP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myelin Basic Protein (MBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
Sensitivity | 5.9 pg/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Myelin Basic Protein (MBP). No significant cross-reactivity or interference between Myelin Basic Protein (MBP) and analogues was observed. | ||||||||||||||||||||||
Publications | Teruya, Shihono et al. Bordetella Dermonecrotic Toxin Is a Neurotropic Virulence Factor That Uses CaV3.1 as the Cell Surface Receptor. mBio. 2020 Mar-Apr 11(2): e03146-19. 32209694 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Description |
Gene Symbol | Mbp |
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Gene Full Name | myelin basic protein |
Alias Symbols | C76307;goll;Golli-Mbp;Hmb;Hmbpr;jv;jve;mld;myelin A1 protein;myelin basic protein;myelin basic protein transcript variant X5;myelin deficient;R75289;shi;shiverer. |
NCBI Gene Id | 17196 |
Protein Name | Myelin basic protein |
Description of Target | The classic group of MBP isoforms (isoform 4-isoform 13) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. |
Uniprot ID | P04370 |
Protein Accession # | NP_001020416.1 |
Nucleotide Accession # | NM_001025245.1 |
Protein Size (# AA) | 250 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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