MBL ELISA Kit (Mouse) (OKDD00633)

Catalog No: OKDD00633 (Formerly GWB-KBBTE2)

Size:96 Wells

Mouse MBL ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology MBL ELISA Kit (Mouse) (OKDD00633) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for MBL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MBL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MBL captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

  Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

  Mean Intra-Assay: CV<10%

  Mean Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-MBL Microplate: 96 Wells (12 x 8 Well Strips)
  • MBL Lyophilized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X MBL Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates.
• Sensitivity: <0.064 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of MBL. No significant cross-reactivity or interference between MBL and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Mbl1|Mbl2
• Gene Full Name: mannose-binding lectin (protein A) 1|mannose-binding lectin (protein C) 2
• Alias Symbols: L-MBP;mannan-binding protein;mannose binding lectin (A);mannose binding lectin, liver (C);mannose binding lectin, serum (A);mannose-binding protein A;mannose-binding protein C;MB;MBL;MBL-;MBL-A;MBL-C;MBP;MBP-;MBP-A;MBP-C;RA-reactive factor P28A subunit;ra-reactive factor polysaccharide-binding component p28B;raRF p28B;RARF/P28A;S-MBP.
• NCBI Gene Id: 17194|17195
• Protein Name: Mannose-binding protein C
• Description of Target: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity).
• Uniprot ID: P41317
• Protein Accession #: NP_034905.1
• Nucleotide Accession #: NM_010775.2
• Protein Size (# AA): 239
• Molecular Weight: 25 kDa