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Catalog No: OKCD02634
Size:96WELLS
Price: $725.00
SKU
OKCD02634
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Datasheets/ManualsPrintable datasheet for MAT2a ELISA kit (Rat)
Product Info
Predicted Species ReactivityRat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methionine Adenosyltransferase II Alpha (MAT2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methionine Adenosyltransferase II Alpha (MAT2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methionine Adenosyltransferase II Alpha (MAT2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Methionine Adenosyltransferase II Alpha (MAT2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methionine Adenosyltransferase II Alpha (MAT2a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methionine Adenosyltransferase II Alpha (MAT2a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MAT2A Microplate96 Wells (12 x 8 Well strips)
MAT2A Lyophilized Standard2
100X Biotinylated MAT2A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Methionine Adenosyltransferase II Alpha (MAT2a). No significant cross-reactivity or interference between Methionine Adenosyltransferase II Alpha (MAT2a) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolMAT2A
Gene Full Namemethionine adenosyltransferase 2A
Alias SymbolsSams2
NCBI Gene Id171347
Protein NameS-adenosylmethionine synthase isoform type-2
Description of TargetCatalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.
Uniprot IDP18298
Protein Accession #NP_599178.1
Nucleotide Accession #NM_134351.1
Protein Size (# AA)395
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