MART-1 Antibody - middle region (OAAB07934)

Catalog No: OAAB07934

Size:400ul

MART-1/Melan-A Antibody (Center) (Cat. #OAAB07934) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MART-1/Melan-A antibody detected the MART-1/Melan-A protein (arrow).

MART-1/Melan-A Antibody (Center) (Cat. #OAAB07934) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, FC
• Additional Information: Precautions: MART-1/Melan-A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes
  Cellular Location: Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation
  Tissue Location: Expression is restricted to melanoma and melanocyte cell lines and retina
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MART-1/Melan-A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-60 amino acids from the Central region of human MART-1/Melan-A.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:2000
  WB: 1:1000
  FC: 1:10~50

Target Info

• Gene Symbol: MLANA
• Alias Symbols: MART1, MART-1
• NCBI Gene Id: 2315
• Protein Name: Melanoma antigen recognized by T-cells 1
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q16655
• Protein Accession #: NP_005502.1
• Molecular Weight: 13157 Da