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MAPKAPK5 Antibody (OAAF02533)
Datasheets/Manuals | Printable datasheet for MAPKAPK5 Antibody (OAAF02533) |
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Predicted Species Reactivity | Human|Mouse |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human MAPKAPK5. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | MAPKAPK5 antibody detects endogenous levels of total MAPKAPK5 protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | IHC: 1:50~1:100; ELISA: 1:5000 |
Reference | New L., EMBO J. 17:3372-3384(1998). The MGC Project Team, Genome Res. 14:2121-2127(2004). Sudo T., Biochem. Biophys. Res. Commun. 269:521-525(2000). |
Gene Symbol | MAPKAPK5 |
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Gene Full Name | MAPK activated protein kinase 5 |
Alias Symbols | MAP kinase-activated protein kinase 5;MAPKAP kinase 5;MAPKAPK-5;MAPKAP-K5;mitogen-activated protein kinase-activated protein kinase 5;MK5;MK-5;p38-regulated/activated protein kinase;PRAK. |
NCBI Gene Id | 8550 |
Protein Name | MAP kinase-activated protein kinase 5 |
Description of Target | Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement. |
Uniprot ID | Q8IW41 |
Protein Accession # | NP_003659.2 |
Molecular Weight | 54 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MAPKAPK5 Antibody (OAAF02533)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "MAPKAPK5 Antibody (OAAF02533)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "MAPKAPK5 Antibody (OAAF02533)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MAPKAPK5 Antibody (OAAF02533)"?
This target may also be called "MAP kinase-activated protein kinase 5;MAPKAP kinase 5;MAPKAPK-5;MAPKAP-K5;mitogen-activated protein kinase-activated protein kinase 5;MK5;MK-5;p38-regulated/activated protein kinase;PRAK." in publications.
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What is the shipping cost for "MAPKAPK5 Antibody (OAAF02533)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MAPKAPK5 Antibody (OAAF02533)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MAPKAPK5 Antibody (OAAF02533)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "54 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MAPKAPK5 Antibody (OAAF02533)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MAPKAPK5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MAPKAPK5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MAPKAPK5"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MAPKAPK5"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MAPKAPK5"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MAPKAPK5"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.