MAPK14 ELISA Kit (Human) : 96 Wells (OKEH01909)

Catalog No: OKEH01909

Size:96T

Human MAPK14 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology MAPK14 ELISA Kit (Human) (OKEH01909) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAPK14 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAPK14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAPK14 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.3% (n = 20)
  Mean Inter-assay CV%: < 10.8% (n = 20)
• ELISA Kit Component:
  • MAPK14 Microplate: 96 Wells (12 x 8 Well strips)
  • MAPK14 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated CHIA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.098 ng/mL
• Specificity: Natural and recombinant Human Mitogen-activated protein kinase 14
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MAPK14
• Gene Full Name: mitogen-activated protein kinase 14
• Alias Symbols: CSAID-binding protein;CSBP;CSBP1;CSBP2;CSPB1;cytokine suppressive anti-inflammatory drug binding protein;Cytokine suppressive anti-inflammatory drug-binding protein;EXIP;MAP kinase 14;MAP kinase Mxi2;MAP kinase p38 alpha;MAX-interacting protein 2;mitogen-activated protein kinase 14;mitogen-activated protein kinase p38 alpha;Mxi2;p38;p38 MAP kinase;p38 mitogen activated protein kinase;p38ALPHA;p38alpha Exip;PRKM14;PRKM15;RK;SAPK2A;stress-activated protein kinase 2A.
• NCBI Gene Id: 1432
• Protein Name: Q16539
• Description of Target: Mitogen-activated protein kinase 14
• Uniprot ID: https://www.uniprot.org/uniprot/Q16539
• Protein Accession #: NP_001306.1
• Nucleotide Accession #: NM_001315.2
• Protein Size (# AA): 360
• Molecular Weight: 41 kDa