MAP4K5 Antibody (OAGA00757)

Catalog No: OAGA00757

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Molt-4
12% SDS PAGE
MAP4K5 antibody
OAGA00757 diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using KHS(OAGA00757) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using KHS(OAGA00757) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HepG2, Molt-4, Raji
  Cellular Localization: Cytoplasm
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide contain a sequence corresponding to a region within amino acids 417 and 482 of KHS
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: MAP4K5
• Gene Full Name: Mitogen-activated protein kinase kinase kinase kinase 5
• Alias Symbols: KHS, GCKR, KHS1, MAPKKKK5
• NCBI Gene Id: 11183
• Protein Name: Mitogen-activated protein kinase kinase kinase kinase 5
• Uniprot ID: Q9Y4K4
• Protein Accession #: NP_006566.2
• Molecular Weight: 95 kDa