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Catalog No: OKCD00830 (Formerly GWB-KBBWL5)
Size:96WELLS
Price: $700.00
SKU
OKCD00830
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Datasheets/ManualsPrintable datasheet for MAP4 ELISA Kit (Rat) (OKCD00830)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein 4 (MAP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein 4 (MAP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein 4 (MAP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Microtubule Associated Protein 4 (MAP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microtubule Associated Protein 4 (MAP4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microtubule Associated Protein 4 (MAP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Map4 Microplate96 Wells (12 x 8 Well Strips)
Map4 Lyophilized Standard2 x 20 ng
100X Biotinylated Map4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.123 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Microtubule Associated Protein 4 (MAP4). No significant cross-reactivity or interference between Microtubule Associated Protein 4 (MAP4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMap4
Gene Full Namemicrotubule-associated protein 4
Alias SymbolsMAP-4;microtubule-associated protein 4;Mtap4.
NCBI Gene Id367171
Protein NameMicrotubule-associated protein 4
Description of TargetNon-neuronal microtubule-associated protein. Promotes microtubule assembly (By similarity).
Uniprot IDQ5M7W5
Protein Accession #NP_001019449
Nucleotide Accession #NM_001024278.1
Protein Size (# AA)1057
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