MAP2K1 Antibody (Phospho-Ser298) (OAAF00126)

Catalog No: OAAF00126 (Formerly GWB-ASB274)

Size:100 ug

Western blot analysis of extracts from NIH-3T3 cells treated with PDGF 50ng/ml 20', using MEK1 (Phospho-Ser298) Antibody. The lane on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MEK1 around the phosphorylation site of Ser298.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: MEK1 (Phospho-Ser298) antibody detects endogenous levels of MEK1 only when phosphorylated at serine 298.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; ELISA: 1:1000
• Reference: Seger R., J. Biol. Chem. 267:25628-25631(1992).
  Zheng C.-F., J. Biol. Chem. 268:11435-11439(1993).
  Zheng C.-F., EMBO J. 13:1123-1131(1994).

Target Info

• Gene Symbol: MAP2K1
• Gene Full Name: mitogen-activated protein kinase kinase 1
• Alias Symbols: CFC3;dual specificity mitogen-activated protein kinase kinase 1;ERK activator kinase 1;MAPK/ERK kinase 1;MAPKK 1;MAPKK1;MEK 1;MEK1;MEL;MKK1;PRKMK1;protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1).
• NCBI Gene Id: 5604
• Protein Name: Dual specificity mitogen-activated protein kinase kinase 1
• Description of Target: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
• Uniprot ID: Q02750
• Protein Accession #: NP_002746.1
• Molecular Weight: 43 kDa