Catalog No: OKEH00582
Size:96T
Price: $625.00
SKU
OKEH00582
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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MAGEC1 ELISA Kit (Human) : 96 Wells (OKEH00582)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MAGEC1 ELISA Kit (Human) (OKEH00582) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human MAGEC1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human MAGEC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human MAGEC1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.5% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
ELISA Kit Component
ComponentAmount
MAGEC1 Microplate96 Wells (12 x 8 Well strips)
MAGEC1 Lyophilized Standard2
100X Biotinylated MAGEC1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.065 ng/mL
SpecificityNatural and recombinant Human Melanoma-associated antigen C1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMAGEC1
Gene Full NameMAGE family member C1
Alias Symbolscancer/testis antigen 7.1;cancer/testis antigen family 7, member 1;CT7;CT7.1;MAGE-C1 antigen;melanoma antigen family C, 1;melanoma antigen family C1;melanoma-associated antigen C1.
NCBI Gene Id9947
Protein NameO60732
Description of TargetMelanoma-associated antigen C1
Uniprot IDhttps://www.uniprot.org/uniprot/O60732
Protein Accession #NP_005453.2
Nucleotide Accession #NM_005462.4
Protein Size (# AA)1142
Molecular Weight124 kDa
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